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Featured publications

Nanobodies to Study G Protein-Coupled Receptor Structure and Function. Manglik, A., Kobilka, BK, J Steyaert, J. Annual review of pharmacology and toxicology 57, 19-37 (2017)


Structure and flexibility equip the endosomal Vps34 complex to phosphorylate membranes. Rostislavleva, K., Soler, N., Ohashi, Y., Zhang, L., Pardon, E., Burke, J., Masson, G.R., Johnson, C., Steyaert, J., Ktistakis, N.T., & Williams, R.L. Science 350, aac7365 (2015) 


Structural Insights into μ-Opioid Receptor Activation. Huang, W, Manglik, A., Venkatakrishnan, A.J., Laeremans, T., Feinberg, E., Sanborn, A., kato, H., Livingston, K., Thorsen, T., Kling, R., Granier, G., Gmeiner, P, Traynor, J., Weis, W., Steyaert, J., Dror, R. & Kobilka, B.  Nature  524, 315–321 (2015) 


Activation and allosteric modulation of a muscarinic acetylcholine receptor. Kruse, A.C.,  Ring, A.M., Manglik, A., Hu, J., Hu, K., Eitel, K., Hübner, H., Pardon, E., Valant, V., Sexton, P.M. , Christopoulos, A., Felder, C., Gmeiner, P., Steyaert, J., Weis, W.I., K., Garcia, K., Wess, J., & Kobilka, B.K. Nature 504, 101-106 (2013)  (PDB entries 4MQS 4MQT)


Structure of a bacterial type IV secretion core complex at subnanometre resolutionRivera-Calzada, A., Fronzes, R., Savva, C. G., Chandran, V. Lian, P. W., Laeremans, T., Pardon, E., Steyaert, J., Remaut, H. Waksman, G., & Orlova, E. V. EMBO J 32, 1195 - 1204 (2013)


SbsB structure and lattice reconstruction unveil Ca++ triggered S-layer assembly. Baranova, E., Fronzes, R., Garcia-Pino, A., Van Gerven, N, Papapostolou, D., Péhau-Arnaudet, Pardon, E., Steyaert, J., Howorka, S. and Remaut, H. Nature 487, 119-122 (2012) (PDB entry 4AQ1)


 

Crystal Structure of the b2 Adrenergic Receptor-Gs protein complex. Rasmussen, S.G.F, DeVree, B .T., Zou, Y, Kruse, A.C., Chung, K.Y.,Thian, T.S., Thian, F.S., Chae, P.S., Pardon, E., Calinski, D., Mathiesen, J.M., Shah, S.T.A., Lyons, J.A., Caffrey, M., Gellman, S.H., Steyaert, J., Skiniotis, G., Weis, W., Sunahara, R.K., Kobilka, B.K. Nature 477, 549–555 (2011) (PDB entry 3SN6)

 

See also News in Focus & News & Views, & Nature Highlights of the year 2011 & Cell-signalling work nets chemistry Nobel

 

Structural basis for GABAA receptor potentiation by neurosteroids. Miller, P. S., Scott, S., Masiulis, S., S., De Colibus, L., Pardon, E., Steyaert, J. & Aricescu, A., R. Nature Struct Mol Biol Advance publication on line (2017)


Allosteric coupling from G protein to the agonist binding pocket in GPCRs. DeVree, B., Mahoney, J., Velez-Ruiz, G., Rasmussen, S., Kuszak, A., Edwald, E., Fung, J. J. , Manglik, A., Masureel, M., Du , Y., Matt, R., Pardon, E., Steyaert, J., Kobilka, B. & Sunahara, R. Nature 535, 182-186 (2016). 


Structure of a prokaryotic Prestin homolog reveals the architecture of the SLC26 family. Geertsma, E.R., Chang, Y.-N., Shaik, F.R., Neldner, Y., Pardon, E., Steyaert, J. & Dutzler, R. Nature Struct Mol Biol 22, 803-808 (2015)


Propagation of conformational changes during μ-opioid receptor activation. Sounier, R., Mas, C., Steyaert, J., Laeremans, T., Manglik, A., Huang, W., Kobilka, B., Demene, H. & Granier, G. (2015)  Nature 524, 375–378 (2015) 


Probing the N-terminal β-sheet conversion in the crystal structure of the full-length human prion protein bound to a Nanobody. Abskharon, R.N.N., Giachin, G., Wohlkonig, A., Soror, S.H., Pardon, E., Legname, G. & Steyaert, J. (2014)  J Am Chem Soc 136, 937-944. 


A general protocol for the generation of Nanobodies for structural biology. Pardon, E., Laeremans, T., Triest, S., Rasmussen, S. G. F., Wohlkönig, A., Ruf, A., Muyldermans, S., Hol, W.J.G.,  Kobilka, B.K. & Steyaert, J. (2014)  Nature protocols 9, 674-693. 


Conformational biosensors reveal GPCR signalling from endosomes. Irannejad, R., Tomshine, J. C., Tomshine, J. R., Chevalier, M., Mahoney, J. P., Steyaert, J., Rasmussen, S. G. F., Sunahara, R. K., El-Samad, H., Huang, B. & von Zastrow, M.  Nature 495, 534-538 (2013)

 

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Atomic structure of a nanobody trapped intermediate of β2m amyloidogenesis. Domanska, K., Vanderhaegen, S, Srinivasan , V.,  Pardon, E., Dupeux, F., Marquez,J.A., Gorgetti, S., Stoppini, M., Wyns, L., Bellotti, V. & Steyaert, J.  Proc Natl Acad Sci USA 108, 1314-1319. (2011) (PDB entry 2X89)


Structure of a nanobody-stabilized active state of the β2 adrenoceptor. Rasmussen, S.G.F., Choi, H.-J., Fung, J.-J., Pardon, E., Casarosa, P., Seok Chae, P. , DeVree, B.T. , Rosenbaum, D.M., Thian, F.S., Kobilka, T.S., Schnapp, A., Konetzki, I., Sunahara, R.K., Gellman, S.H., Pautsch, A. , Steyaert, J., Weis, W.I., & Kobilka, B.K. Nature 469, 175-80. (2011) (PDB entry 3P0G)

 

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